In the mouse thymus, newly formed single positive (SP) cells spend an
average of 14 days in the thymic medulla. During this time, phenotypic
and functional maturation occurs with down-regulation of CD69 and hea
t stable antigen (HSA), and up-regulation of Qa-2. Very little is know
n about the final steps that allow or direct these T cells to emigrate
and join the recirculating peripheral T cell pool. Currently availabl
e data suggest that not all recent thymic emigrants (RTE) complete thi
s maturational sequence in the medulla and that emigration may occur a
t any time during the medullary maturation stage. In this study, we ha
ve compared adhesion and activation marker expression on SP thymocytes
, RTE and peripheral T cells to determine more precisely which SP medu
llary thymocytes are exported. Although RTE were heterogeneous for HSA
and Qa-2 expression, they were quite uniform with regard to the expre
ssion of other molecules. In contrast to medullary SP thymocytes, most
RTE were L-selectin(high) and CD69(-). In addition, CD4(+) CD8(-) and
CD4(-) CD8(+) RTE were phenotypically distinct from each other in tha
t the former were beta(7) integtin(-/low), CD45RB(intermediate) and CD
45RC(-), while the latter were beta(7) integrin(high), CD45RB(high) an
d CD45RC(low). These phenotypes were comparable to only a minor (as li
ttle as 6 %) subpopulation of medullary SP thymocytes. Overall, the da
ta indicate that export of cells from the medullary pool of SP thymocy
tes is not random, but that a series of maturational events within the
SP stage are necessary before export can occur.