Analysis of diversity within Fusarium oxysporum populations using molecular and vegetative compatibility grouping

Citation
Jl. Smith-white et al., Analysis of diversity within Fusarium oxysporum populations using molecular and vegetative compatibility grouping, AUSTRALAS P, 30(2), 2001, pp. 153-157
Citations number
21
Categorie Soggetti
Plant Sciences
Journal title
AUSTRALASIAN PLANT PATHOLOGY
ISSN journal
08153191 → ACNP
Volume
30
Issue
2
Year of publication
2001
Pages
153 - 157
Database
ISI
SICI code
0815-3191(2001)30:2<153:AODWFO>2.0.ZU;2-7
Abstract
DNA fingerprints, based on Enterobacterial Repetitive Intergenic Consensus (ERIC) sites, were investigated as an alternative technique to Vegetative C ompatibility Grouping (VCG) in studying populations of Fusarium oxysporum S chlecht. emend. Snyd. & Hans. Isolates of F. oxysporum were recovered from orchids displaying visible signs of root rot. Fifty-two isolates were colle cted and analysed using both the ERIC DNA fingerprinting technique and Vege tative Compatibility Grouping. Isolates were divided into 17 ERIC groups an d 37 VCG groups. Based on its lower level of sensitivity and its rapid and reproducible result, the ERIC DNA fingerprinting was found to be a more app ropriate technique for studying diverse populations of F. oxysporum.