Analysis of the mouse Scnn1a promoter in cortical collecting duct cells and in transgenic mice

Authors
Kohler, S Pradervand, S Verdumo, C Merillat, AM Bens, M Vandewalle, A Beermann, F Hummler, E
Citation
S. Kohler et al., Analysis of the mouse Scnn1a promoter in cortical collecting duct cells and in transgenic mice, BBA-GENE ST, 1519(1-2), 2001, pp. 106-110
Citations number
19
Categorie Soggetti
Molecular Biology & Genetics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
ISSN journal
01674781 → ACNP
Volume
1519
Issue
1-2
Year of publication
2001
Pages
106 - 110
Database
ISI
SICI code
0167-4781(20010528)1519:1-2<106:AOTMSP>2.0.ZU;2-8
Abstract
We have isolated and characterised the promoter of the mouse Scnn1a (alpha ENaC) gene. Using transient transfections of serial deletion mutants into S cnn1a-expressing cells, we demonstrate that 1.56 kb of 5' upstream sequence is required for cell-specific expression and corticosteroid-mediated regul ation. These 5' sequences are not sufficient to drive expression of a laci! reporter gene or a rat Scnn1a cDNA in transgenic mice, where they failed t o rescue Scnn1a deficiency. (C) 2001 Elsevier Science B.V. All rights reser ved.