Developmental program expression of myosin alkali light chain and skeletalactin genes in the rainbow trout Oncorhynchus mykiss

Citation
P. Thiebaud et al., Developmental program expression of myosin alkali light chain and skeletalactin genes in the rainbow trout Oncorhynchus mykiss, BBA-GENE ST, 1519(1-2), 2001, pp. 139-142
Citations number
15
Categorie Soggetti
Molecular Biology & Genetics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
ISSN journal
01674781 → ACNP
Volume
1519
Issue
1-2
Year of publication
2001
Pages
139 - 142
Database
ISI
SICI code
0167-4781(20010528)1519:1-2<139:DPEOMA>2.0.ZU;2-D
Abstract
We have isolated MLC1(F) (tMLC1(F)), MLC3(F) (tMLC3(F)) and skeletal actin cDNAs from the teleost Oncorhynchus mykiss. Sequence analysis indicates tha t tMLCl(F) and tMLC3F are not produced from differentially spliced mRNAs as reported in avians and rodents but are encoded by different genes. Results from RNase protection analysis showed that the corresponding transcripts a re expressed in fast skeletal muscles. Whole-mount in situ hybridisation re vealed distinct expression patterns of the myosin alkali light chains and s keletal actin genes during skeletal muscle development in the embryo. (C) 2 001 Elsevier Science B.V. All rights reserved.