Some features of intestinal absorption of intact fibrinolytic enzyme III-1from Lumbricus rubellus

Citation
Q. Fan et al., Some features of intestinal absorption of intact fibrinolytic enzyme III-1from Lumbricus rubellus, BBA-GEN SUB, 1526(3), 2001, pp. 286-292
Citations number
29
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
ISSN journal
03044165 → ACNP
Volume
1526
Issue
3
Year of publication
2001
Pages
286 - 292
Database
ISI
SICI code
0304-4165(20010615)1526:3<286:SFOIAO>2.0.ZU;2-E
Abstract
In order to investigate whether earthworm fibrinolytic enzyme III-1 (EFE-II I-1) isolated from Lumbricus rubellus is capable of transporting into blood through intestinal epithelium and keeping its biological function in circu lation, we have raised an antibody against EFE-III-I. The immunological res ults showed that 10-15% of intact EFE-III-I was absorbed by the intestinal epithelium with the incubation chamber method [Vilhardt and Lundin, Acta Ph ysiol. Scand. 126 (1986) 601-607]. The enzyme could be detected in the inte stinal epithelial cells by immunohistochemistry. Furthermore, immunoreactiv e intact EFE-III-I was found in serum or plasma after intraperitoneal injec tion of rats. Approx. 10% of the full-size enzyme could transport through t he intestinal epithelium. The maximum remaining activity in blood could be assayed around 60 min after the intraperitoneal injection. (C) 2001 Elsevie r Science B.V. All rights reserved.