CONVERSION OF OLEIC-ACID TO 10-HYDROXYSTEARIC ACID BY NOCARDIA-PARAFFINAE

Authors
LATRASSE A PAITIER S LACHOT B BONNARME P FERON G DURAND A LEQUERE JL
Citation
A. Latrasse et al., CONVERSION OF OLEIC-ACID TO 10-HYDROXYSTEARIC ACID BY NOCARDIA-PARAFFINAE, Biotechnology letters, 19(8), 1997, pp. 715-718
Citations number
12
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
19
Issue
8
Year of publication
1997
Pages
715 - 718
Database
ISI
SICI code
0141-5492(1997)19:8<715:COOT1A>2.0.ZU;2-L
Abstract
Resting cells of Nocardia paraffinae CBS 255 58 anaerobically converte d oleic acid to 10-hydroxystearic acid (optimum activity at 35 degrees C and pH 7). Addition of oleic acid (0.22 mg/ml of medium), as an ind ucer, improved the hydroxylating activity. The biocatalyst was inactiv ated by sonication, lyophilization and heating, but kept 65% of its in itial activity after five freeze-thawing treatments. This activity app eared to be located either in the cells or bound to the plasmic membra ne.