NAD(-DEHYDROGENASE IN THE PRESENCE OF PYRAZOLE AND A NEW METHOD FOR THE DETERMINATION OF THE ENZYME ACTIVE-SITE CONCENTRATION() BINDING BY YEAST ALCOHOL)

Authors
TRIVIC S ZEREMSKI J LESKOVAC V
Citation
S. Trivic et al., NAD(-DEHYDROGENASE IN THE PRESENCE OF PYRAZOLE AND A NEW METHOD FOR THE DETERMINATION OF THE ENZYME ACTIVE-SITE CONCENTRATION() BINDING BY YEAST ALCOHOL), Biotechnology letters, 19(8), 1997, pp. 809-811
Citations number
7
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
19
Issue
8
Year of publication
1997
Pages
809 - 811
Database
ISI
SICI code
0141-5492(1997)19:8<809:NITPOP>2.0.ZU;2-2
Abstract
The pyrazole method of Theorell and Yonetani (Biochem. Z., 338: 573-55 3, 1963) has been adapted for the determination of the enzyme active s ite concentration in yeast alcohol dehydrogenase (EC 1.1.1.1). This me thod cannot be applied indiscriminately to other alcohol dehydrogenase s without modification.