Incorporation of alpha- and beta-LNA (locked nucleic acid) monomers in oligodeoxynucleotides with polarity reversals

Authors
Christensen, NK Dahl, BM Nielsen, P
Citation
Nk. Christensen et al., Incorporation of alpha- and beta-LNA (locked nucleic acid) monomers in oligodeoxynucleotides with polarity reversals, BIOORG MED, 11(13), 2001, pp. 1765-1768
Citations number
21
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
ISSN journal
0960894X → ACNP
Volume
11
Issue
13
Year of publication
2001
Pages
1765 - 1768
Database
ISI
SICI code
0960-894X(20010709)11:13<1765:IOAAB(>2.0.ZU;2-A
Abstract
The thymidine monomers of LNA with both alpha- and beta -configuration are incorporated with polarity reversals (i.e., with 3'-3' and 5'-5' junctions) in oligodeoxynucleotides with beta- and alpha -configuration, respectively . A 5'-O-phosphoramidite of the beta -LNA monomer is synthesised. Large des tabilisations of duplexes with both complementary DNA and RNA are observed for oligodeoxynucleotides containing the alpha -LNA monomer, whereas a dupl ex with complementary RNA of an alpha -oligodeoxynucleotide containing the beta -LNA monomer is not destabilised. (C) 2001 Elsevier Science Ltd. All r ights reserved.