Yw. Chen et al., BCL10 somatic mutations rarely occur in gastric lymphoma: detection of high frequency of polymorphisms in BCL10 coding region, CANC GENET, 127(2), 2001, pp. 184-187
The BCL10 gene, recently isolated due to its involvement in the t(l:14)(p22
:q32) of mucosa-associated lymphoid tissue B cell non-Hodgkin lymphoma (MAL
Toma). was shown to have frequent somatic mutations and short deletions wit
hin the coding region in MALToma and a variety of other lymphomas and solid
tumors. These observations have been recently questioned. In this study, w
e examined BCL10 gene mutations by direct sequencing of the entire coding r
egion of the BCL10 gene, amplified from paired normal and tumor genomic DNA
s, as well as tumor cDNAs. in 23 cases of primary gastric B cell non-Hodgki
n lymphomas. comprising of 6 cases of MALToma and 17 cases of diffuse large
cell (DLC) lymphoma. Heterozygosity due to three types of known polymorphi
sms in codon 5 (17.3%), codon 8 (21.7%), and codon 213 (8.6%) were observed
in both normal germline DNA and tumor DNAs and tumor cDNAs in individual c
ases. In one case (4.3%) GIC heterozygosity in codon 8 in normal germline D
NA was reduced to homozygosity (LOH) in tumor DNA and cDNA. Mutations inact
ivating BCL10 gene product function were not found in any of these cases. M
oreover. post-transcriptional alterations were not indicated by abnormaliti
es in BCL10 mRNA sequence in tumor cDNAs in these gastric lymphoma cases. O
ur results show that somatic mutations in the BCL10 gene rarely occur in ga
stric lymphoma and indicate that this gene is unlikely to be of pathogeneti
c significance in the majority of gastric lymphomas. (C) 2001 Elsevier Scie
nce Inc. All rights reserved.