One way to promote chromosome pairing between wheat and alien genomes is by
using chromosome 5B deficient-genetic stocks (monosomic 5B) as female pare
nts in hybridization. Such a stock is available in the cultivar Chinese Spr
ing that has superior crossability but is a poor agronomic wheat. We have d
eveloped monosomic 5B stocks in three elite bread wheat cultivars (Ciano 79
, Nacozari 75, Pavon 76) using Chinese Spring as the monosomic 5B donor sou
rce through a backcrossing protocol. The three new monosomic 5B stocks prod
uced were validated by C-banding and by meiotic analyses of the 5B deficien
t F-1 hybrids (2n=5x=34) of each cultivar with Aegilops variabilis.