Design of histidine-Zn2+ binding sites within a beta-hairpin peptide: enhancement of beta-sheet stability through metal complexation

Authors
Platt, G Chung, CW Searle, MS
Citation
G. Platt et al., Design of histidine-Zn2+ binding sites within a beta-hairpin peptide: enhancement of beta-sheet stability through metal complexation, CHEM COMMUN, (13), 2001, pp. 1162-1163
Citations number
21
Categorie Soggetti
Chemistry
Journal title
CHEMICAL COMMUNICATIONS
ISSN journal
13597345 → ACNP
Issue
13
Year of publication
2001
Pages
1162 - 1163
Database
ISI
SICI code
1359-7345(2001):13<1162:DOHBSW>2.0.ZU;2-F
Abstract
We describe the design and characterisation of two simple 'metalloproteins' based on Zn2+ co-ordination sites involving histidine residues close to th e N- and C-termini of two beta -hairpin peptides (His(2)-beta, KHYTVSINGKKI TVHI and His(3)-beta, HKHYTV-SINGKKITVHI); we show by NMR and circular dich roism spectroscopy that Zn2+ complexation co-operatively enhances the stabi lity of these partially pre-organised beta -sheet peptides.