Enzymatic aqueous extraction of soybean oil

The procedure of enzymatic aqueous extraction of soybean oil was assessed w hen two-step controlled enzymatic hydrolysis was applied. With aqueous extr action of soybean oil-containing protein, the highest yield of oil was 96.1 % at the optimized conditions studied. Soybean oil-containing protein was h ydrolyzed and resulted in releasing part of oil. The separated protein that contained 40% oil was enriched due to its adsorption capacity of released oil, the average oil extraction yeild reached 93.5%. Then the high oil cont ent protein was hydrolyzed again to release oil by enzyme, the oil extracti on yeild was 80.4%. As a result, high quality of soybean oil was obtained a nd the content of total oil yield was 74.4%.