J. Dionne et al., Freezing tolerance and carbohydrate changes during cold acclimation of green-type annual bluegrass (Poa annua L.) ecotypes, CROP SCI, 41(2), 2001, pp. 443-451
Winterkill is recurrently observed on annual bluegrass (Poa annua L,) golf
greens in northern climates. Although annual bluegrass susceptibility to fr
eezing temperatures has been pointed out as a major factor responsible for
minter damages, little information exists on freezing tolerance and cold ha
rdening of green-type annual bluegrass. This study was conducted to assess
freezing tolerance and carbohydrate changes occurring during cold acclimati
on of green-type annual bluegrass ecotypes cold hardened under both environ
mentally controlled and simulated winter conditions in an unheated greenhou
se. The 50% killing temperatures (LT50), levels of fructans, and mono and d
isaccharides were determined during cold acclimation in three annual bluegr
ass ecotypes originating from Western Pennsylvania (OK), Coastal Margland (
CO) and Central Quebec (CR), The ecotypes differed significantly with regar
d to their freezing tolerance (LT50 ranking: OK < CO < CR) and maintained t
heir relative ranking under both environmentally controlled and simulated-n
atural winter conditions, Maximum freezing tolerance was observed after exp
osure to nonlethal subfreezing temperatures and annual bluegrass achieved h
igh levels of freezing tolerance with LT50 of -31.2 degreesC for OK, -24.6
degreesC for CO, and -22.8 degreesC for CR. High molecular weight fructans
(DP >6) were the most abundant carbohydrates found in plants cold-acclimate
d under low, nonfreezing temperature with levels up to 170 mg g(-1) dry wei
ght as compared with 60 to 70 mg g(-1) dry weight in nonacclimated plants.
Sucrose levels in crowns of annual bluegrass markedly increased at temperat
ures below freezing and maximum sucrose concentration coincided with maximu
m freezing tolerance of annual bluegrass, However, variations in fructan an
d sucrose levels were not related to differential freezing tolerance among
the three annual bluegrass ecotypes tested.