Constant-current-mode LAPS (CLAPS) for the detection of penicillin

Authors
Yoshinobu, T Ecken, H Poghossian, A Simonis, A Iwasaki, H Luth, H Schoning, MJ
Citation
T. Yoshinobu et al., Constant-current-mode LAPS (CLAPS) for the detection of penicillin, ELECTROANAL, 13(8-9), 2001, pp. 733-736
Citations number
17
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ELECTROANALYSIS
ISSN journal
10400397 → ACNP
Volume
13
Issue
8-9
Year of publication
2001
Pages
733 - 736
Database
ISI
SICI code
1040-0397(200105)13:8-9<733:CL(FTD>2.0.ZU;2-#
Abstract
A software feedback control system for the constant-current-mode operation of the light-addressable potentiometric sensor (LAPS) was developed. The co nstant-current-mode LAPS (CLAPS) is suitable for online monitoring and reco rding of changes in the pH value or the ion concentration. An enzyme LAPS w as fabricated by adsorptive immobilization of penicillinase on the pH-sensi tive layer of Ta2O5 This sensor was operated in the constant-current-mode a nd the detection limit for penicillin G was found to be at least as low as 100 muM.