Telomere resolution in the Lyme disease spirochete

Citation
G. Chaconas et al., Telomere resolution in the Lyme disease spirochete, EMBO J, 20(12), 2001, pp. 3229-3237
Citations number
40
Categorie Soggetti
Molecular Biology & Genetics
Journal title
EMBO JOURNAL
ISSN journal
02614189 → ACNP
Volume
20
Issue
12
Year of publication
2001
Pages
3229 - 3237
Database
ISI
SICI code
0261-4189(20010615)20:12<3229:TRITLD>2.0.ZU;2-J
Abstract
The genus Borrelia includes the causative agents of Lyme disease and relaps ing fever. An unusual feature of these bacteria is a genome that includes l inear DNA molecules with covalently closed hairpin ends referred to as telo meres, We have investigated the mechanism by which the hairpin telomeres ar e processed during replication. A synthetic 140 bp sequence having the pred icted structure of a replicated telomere was shown to function as a viable substrate for telomere resolution in vivo, and was sufficient to convert a circular replicon to a linear form. Our results suggest that the final step in the replication of linear Borrelia replicons is a site-specific DNA bre akage and reunion event to regenerate covalently closed hairpin ends. The t elomere substrate described here will be valuable both for in vivo manipula tion of linear DNA in Borrelia and for in vitro studies to identify and cha racterize the telomere resolvase.