Samui, a novel cold-inducible gene, encoding a protein with a BAG domain similar to silencer of death domains (SODD/BAG-4), isolated from Bombyx diapause eggs
Y. Moribe et al., Samui, a novel cold-inducible gene, encoding a protein with a BAG domain similar to silencer of death domains (SODD/BAG-4), isolated from Bombyx diapause eggs, EUR J BIOCH, 268(12), 2001, pp. 3432-3442
Cellular responses to cold-acclimation have not yet been studied in depth.
To explore this field, we focussed on insect diapause development. Although
embryonic diapause of Bombyx mori is sustained at 25 degreesC, chilling at
5 degreesC for 2 months causes diapause termination, a transition that is
marked when the sorbitol dehydrogenase gene (SDH) is activated. To clarify
the relationship between this activation and incubation at 5 degreesC, we i
solated a novel cold-inducible gene, Samui. Expression of Samui mRNA and pr
otein was activated after incubation at 5 degreesC for 5-6 days, lasted for
another 30 days and then weakened. Exposure to 25 degreesC suppressed both
mRNA and protein expression. In nondiapause eggs incubated at 5 degreesC,
Samui was also up-regulated, although the expression was weaker. Samui cont
ained nuclear localization-signals, a ssDNA-binding motif and a BAG domain
similar to that of SODD/BAG-4. Because Samui could bind to HSP70, it is a m
ember of BAG protein family. It is proposed that Samui serves to transmit t
he '5 degreesC signal' for SDH expression in diapause eggs, while also prot
ecting against cold-injures in nondiapause eggs, through binding to respect
ive partners. This is the first report that a member of BAG protein family
is up-regulated by cold.