Agitation, temperature, inoculum size, initial pH and pH of buffered medium
affected the decolorization of Orange II dye by Coriolus versicolor and Fu
nalia trogii. The optimum temperature and initial pH value for decolorizati
on were 30 degreesC and 6.5-7.0, respectively; pH 4.5 was the most efficien
t in buffered cultures. High decolorization extents were reached at all agi
tation rates. At an inoculum size of more than 1 mt, the extent of decolori
zation changed only slightly. High extents were obtained using immobilized
fungi at repeated batch mode.