Restricted V-H gene usage in lamina propria B cells producing anticolon antibody from patients with ulcerative colitis

Background & Aims: Autoimmune responses against colonic epithelium may play a role in the development of colonic inflammation associated with ulcerati ve colitis (UC). In this study, we established and characterized B-cell lin es and clones that produced anticolon antibody from inflamed colonic mucosa of UC subjects. Methods: B-cell lines were generated through Epstein-Barr virus transformation of lamina propria lymphocytes (LPLs) from colonic muco sa and peripheral blood lymphocytes, and these lines were screened for the production of anticolon antibodies. B-cell lines were then cloned by limiti ng dilution culture, and messenger RNA expression of immunoglobulin heavy-c hain variable region (V-H) was assessed. Results:V-H gene families used in B-cell lines established from LPLs of normal controls were diverse, and B-c ell lines from UC LPLs expressed a restricted V(H)3 family usage. All 15 cl ones from UC used a restricted VH3 gene family, whereas diverse V-H gene fa milies were used by 24 clones from normal controls. The analysis of nucleot ide sequences indicated that these clones were derived from various germlin e gene segments. Conclusions: The restricted V-H gene usage in anticolon au toantibodies producing B-cell clones suggests that a particular antigenic s timulus contributes to the pathogenesis of UC.