Genetic polymorphisms of biotransformation enzymes in patients with Hodgkin's and non-Hodgkin's lymphomas

Considering the role in the metabolism of chemicals played by biotransforma tion enzymes, we aimed at determining whether any association exists betwee n genetic polymorphisms in CYP1A1, CYP2E1,epoxide hydrolase (EPHX), glutath ione S-transferases (GSTM1/P1/T1) and individual susceptibility to lymphoma s, PCR-RFLP-based genotyping assays were used to determine the frequency of polymorphisms in CYP1A1(3 ' -flanking region), CYP2E1 (5 ' -flanking regio n and intron 6), EPHX (exons 3 and 4), GSTM1 (deletion), GSTP1 (exon 5) and GSTT1 (deletion) in a case-control study comprised of 219 patients with mo rbus Hodgkin (MH) and non-Hodgkin's lymphomas (NHL) and 455 age- and sex-ma tched healthy individuals. The distribution of genotypes in CYP2E1-intron 6 was significantly different between the control group and all lymphomas (P = 0.03), patients with NHL (P = 0.024), and especially aggressive diffuse NHL (P = 0.007), Grading of NHL seemed to be associated with this polymorph ism as well (P = 0.041), The EPHX-exon 3 genotype distribution was signific antly different between control males and males with all lymphomas (P = 0.0 1) or with NHL (P = 0.019), The Val/Val genotype of GSTP1-exon 5 was preval ent in all MH [odds ratio (OR) = 2,08, 95% confidence interval (CI) = 1.05- 4.14] and this difference was particularly evident in females (OR = 2,97, 9 5% CI = 1.16-7.61), A significant difference in the distribution of GSTP1-e xon 5 genotypes was found between NHL tumors >5 cm and those <5 cm (P = 0.0 3), The results suggest that genetic polymorphisms of biotransformation enz ymes may play a significant role in the development of lymphoid malignancie s.