The relationship between salt homeostasis and blood pressure has remained d
ifficult to establish from epidemiological studies of the general populatio
n. Recently, mendelian forms of hypertension have demonstrated that mutatio
ns that increase renal salt balance lead to higher blood pressure, suggesti
ng that mutations that decrease the net salt balance might have the convers
e effect. Gitelman's syndrome, caused by loss of function mutations in the
Na-Cl cotransporter of the distal convoluted tubule (NCCT), features inheri
ted hypokalemic alkalosis with so-called "normal" blood pressure. We hypoth
esized that the mild salt wasting of Gitelman's syndrome results in reduced
blood pressure and protection from hypertension. We have formally addresse
d this question through the study of 199 members of a large Amish kindred w
ith Gitelman's syndrome. Through genetic testing, family members were ident
ified as inheriting 0 (n=60), 1 (n=113), or 2 (n=26) mutations in NCCT, per
mitting an unbiased assessment of the clinical consequences of inheriting t
hese mutations by comparison of the phenotypes of relatives with contrastin
g genotypes. The results demonstrate high penetrance of hypokalemic alkalos
is, hypomagnesemia, and hypocalciuria in patients inheriting 2 mutant NCCT
alleles. In addition, the NCCT genotype was a significant predictor of bloo
d pressure, with homozygous mutant family members having significantly lowe
r age- and gender-adjusted systolic and diastolic blood pressures than thos
e of their wild-type relatives. Moreover, both homozygote and heterozygote
subjects had significantly higher 24-hour urinary Na+ than did wild-type su
bjects, reflecting a self-selected higher salt intake. Finally, heterozygou
s children, but not adults, had significantly lower blood pressures than th
ose of the wild-type relatives. These findings provide formal demonstration
that inherited mutations that impair renal salt handling lower blood press
ure in humans.