S. Kasai et Jg. Scott, Cytochrome P450s CYP6D3 and CYP6D1 are part of a P450 gene cluster on autosome 1 in the house fly, INSEC MOL B, 10(3), 2001, pp. 191-196
The P450 monooxygenases of insects are important in the metabolism of numer
ous endogenous and exogenous compounds. However, identity of the P450 isofo
rm(s) involved in these reactions is rarely known. A critical first step in
the identification of important P450s is the cloning and sequencing of the
ir genes. Toward this goal we report the genomic sequence of a new cytochro
me P450, termed CYP6D3, from the house fly, Musca domestica. CYP6D3 is part
of a P450 gene cluster located on chromosome 1 and is located upstream of
a related gene, CYP6D1. The similar genetic structures of CYP6D3 and CYP6D1
(5 exons and 4 introns of similar length) suggest one of these genes may h
ave been the result of a duplication event. The CYP6D3 deduced amino acid s
equence indicates a protein with 518 amino acids and a molecular weight of
59.3 kDa. The CYP6D3 protein is most similar to house fly CYP6D1 (78%) and
Cyp6D2 (56%) from Drosophila melanogaster. The deduced amino acid sequences
of CYP6D3 and CYP6D1 are identical at the Helix I and heme binding regions
.