H. Tanaka et al., Acute myelogenous leukemia with PIG-A gene mutation evolved from aplastic anemia-paroxysmal nocturnal hemoglobinuria syndrome, INT J HEMAT, 73(2), 2001, pp. 206-212
We report a patient with aplastic anemia (AA)-paroxysmal nocturnal hemoglob
inuria (PNH) syndrome who developed acute myelogenous leukemia (AML). Flow
cytometric analysis showed that the leukemic cells in the bone marrow lacke
d CD59 antigen on their surface and were positive for P-glycoprotein. Heter
oduplex and single-strand conformation polymorphism analysis followed by se
quencing of the leukemic cells in the bone marrow disclosed 1 frameshift-ty
pe mutation in exon 2 of the phosphatidylinositol glycan-class A (PIG-A) ge
ne, which deductively produces truncated PIG-A protein. These findings prov
ide direct evidence that the leukemic cells evolved from the affected PNH c
lone. Cytogenetic analysis in the bone marrow in each stage of AA-PNH. AML,
and at relapse of AML showed normal, -7, and -7 plus -20, respectively, sh
owing evidence of a clonal evolution. Because complete remission of AML was
not achieved by intensive chemotherapies, allogeneic peripheral blood stem
cell transplantation (PBSCT) from the patient's HLA-matched sister was per
formed successfully with recovery of CD59 antigen on bone marrow hematopoie
tic cells: however, leukemia relapsed 4 months after PBSCT. Leukemia derive
d from PNH may be resistant to intensive chemotherapy, and a highly myeloab
lative regimen may be required for stem cell transplantation to eradicate t
he PNH-derived leukemia clone. (C) 2001 The Japanese Society of Hematology.