Inhibitory effect of lactoperoxidase-generated hypothiocyanite upon black pigmented anaerobe growth

This study aimed to evaluate the in vitro effect of lactoperoxidase with or without its substrates (hydrogen peroxide, thiocyanate) on the growth of 4 different black pigmented anaerobe (BPA) strains associated with the devel opment and progress of periodontal diseases: Porphyromonas gingivalis ATCC 33277, Prevotella intermedia NCTC 9336, Prevotella loescheii ATCC 15930, an d Prevotella melaninogenica NCTC 9338. A 5-min lactoperoxidase-generated OS CN--HOSCN incubation at pH 6.0, 7.0 or 8.0 resulted in a decrease of the gr owth rate (tested by turbidimetry in liquid cultures) of the 4 BPA strains, whilst lactoperoxidase alone actually promoted bacterial growth.