Citrus pectin: Characterization and inhibitory effect on fibroblast growthfactor-receptor interaction

This study was undertaken to characterize the pectin from four citrus speci es and to determine their in vitro inhibitory activities on the binding of fibroblast growth factor (FGF) to the FGF receptor (FGFR). Pectin from vari ous parts of lemon, grapefruit, tangerine, and orange were isolated and cha racterized Tangerine had the highest pectin content among the four citrus s pecies. Segment membrane contained as much as or more pectin than flavedo/a lbedo. Anhydrogalacturonic content was highest in pectin from segment membr ane of tangerine and flavedo/albedo of grapefruit. Lemon pectin contained t he highest methoxyl content (MC), and grapefruit contained the largest prop ortion of lower molecular weight (< 10000 Da) pectin. Tangerine contained t he highest neutral sugar in both flavedo/albedo and segment membrane. The i nterdependency of heparin on factor-receptor interaction provides a means f or identifying new antagonists of growth factor activity and thus for treat ment of various diseases. These results showed that pectin significantly in hibited the binding of FGF-1 to FGFR1 in the presence of 0.1 mug/mL heparin . The pectin from the segment membrane of lemon was the most potent inhibit or. The inhibition activity was significantly correlated with sugar content , MC, and size of pectin. Kinetic studies revealed a competitive nature of pectin inhibition with the heparin, a crucial component of the FGF signal t ransduction process. The observation that the heparin-dependent biological activity of FGF signal transduction is antagonized by citrus pectin should be further investigated for the use of these pectins as anti-growth factor agents for potential health benefits.