Background: Exposure to occupational agents can induce eosinophilic inflamm
ation in subjects with occupational asthma (OA), It might also induce nonsp
ecific changes in airway inflammation in subjects without OA,
Objectives: We sought to investigate the changes in airway inflammation ind
uced by exposure to occupational agents in subjects with and without OA and
to determine which changes in sputum eosinophil numbers and bronchial resp
onsiveness to methacholine should be regarded as clinically significant for
predicting a 20% fall in FEV1.
Methods: We performed specific inhalation challenges (SICs) in 3 groups of
subjects: subjects reporting a history consistent with OA with a positive S
IC response (n = 17); subjects reporting a history consistent with OA with
a negative SIC response (n = 14); and asthmatic subjects without any histor
y of OA (n = 10). Induced sputum and methacholine challenges were performed
at the end of the control day and again at the end of the last day of expo
sure; the last day of exposure was always performed in the laboratory.
Results: There was an increase in median sputum eosinophil and neutrophil n
umbers in subjects with positive SIC responses. Cell counts remained unchan
ged after exposure in asthmatic subjects without OA, A combination of a gre
ater than 0.26 10(6)/mL increase in sputum eosinophil numbers and a decreas
e in the concentration of methacholine inducing a 20% fall in FEV1 of at le
ast 1.8-fold compared with baseline values predicted a 20% fall in FEV1 in
96% (95% CI, 70%-99%) of patients.
Conclusion: Exposure to occupational agents per se does not induce airway i
nflammation. Changes in both sputum eosinophil counts and methacholine resp
onsiveness are satisfactory predictors of a significant bronchial responsiv
eness to occupational agents.