Enhancing detection sensitivity in capillary electrophoresis

Authors
Mosulishvili, LM Barnov, VA Tsibakhashvili, NY Engelhardt, H Beck, W
Citation
Lm. Mosulishvili et al., Enhancing detection sensitivity in capillary electrophoresis, J ANALYT CH, 56(6), 2001, pp. 512-514
Citations number
14
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF ANALYTICAL CHEMISTRY
ISSN journal
10619348 → ACNP
Volume
56
Issue
6
Year of publication
2001
Pages
512 - 514
Database
ISI
SICI code
1061-9348(200106)56:6<512:EDSICE>2.0.ZU;2-F
Abstract
A simple technique was proposed for improving detection sensitivity in capi llary electrophoresis by the chemical etching of the interior surface of a fused-silica capillary in the detection region. This technique was applied to the electrophoresis of proteins. The sensitivity was found to increase, on the average, hy a factor of 4 to 5 after etching a 75-mum i.d. capillary with an HF solution for 7 min.