Flow-injection determination of benzylpenicillin novocaine salt in penicillin preparations with spectrophotometric detection

Working conditions were found for determining the novocaine salt of benzylp enicillin antibiotic as its 4,6-dinitrobenzofuroxan derivative by flow-inje ction analysis with spectrophotometric detection (lambda = 510 nm). The bes t results were obtained for Bows of methanol (ethanol) and water with a vol ume ratio of 30 : 70. The calibration range of the pharmaceutical is 0.28-1 0 mug/mL with a throughput capacity of 30 samples/h. The limit of detection (3 sigma, n = 4) is 0.14 mug/mL. Amino acids and components of penicillin- containing pharmaceuticals did not interfere with the determination. Proced ures for determining benzylpenicillin novocaine salt in medicinal formulati ons containing benzylpenicillin N,N ' -dibenzylethylenediamine, potassium, and sodium salts and some other.