Ultrasound measurements of fetal breathing movements in the rat

The goal of this study was to determine when fetal breathing movements (FBM s) commence in the rat and to characterize age-dependent changes of FBMs in utero. These data provide a frame of reference for parallel in vitro studi es of the cellular, synaptic, and network properties of the perinatal rat r espiratory system. Ultrasound recordings were made from unanesthetized Spra gue-Dawley rats from embryonic (E) day 15 (E15) to E20. Furthermore, the ef fects of respiratory stimulants (doxapram and aminophylline) and hypoxia on FBMs were studied. Single FBMs, occurring at a very low frequency (similar to8 FBMs/h), commenced at E16. The incidence of single FBMs increased to s imilar to 80 FBMs/h by E20. Episodes of clustered rhythmic FBMs were first observed at E18 (similar to 40 FBMs/h). The incidence of episodic clustered FBMs increased to similar to 300 FMBs/h by E20, with the duration of each episode ranging from similar to 40 to 180 s. Doxapram, presumably acting to stimulate carotid body receptors, did not increase FBMs until E20, when th e incidence of episodic clustered FBMs increased twofold. Aminophylline, a central-acting stimulant, caused an increase in episodic clustered FBMs aft er E17, reaching significance at E20 (3-fold increase). Exposing the dam to 10% O-2 caused a rapid, marked suppression of FBMs (5-fold decrease) that was readily reversed on exposure to room air.