Relation between different human sperm nuclear maturity tests and in vitrofertilization

Purpose: To consider the relationship between different sperm nuclear matur ity tests and in vitro fertilization (IVF) rare, in order to select the mos t sensitive, specific, and independent factor(s) for prediction of in vitro fertilization. Methods Infertile couples (101) were randomly selected from IVF candidates referred to Isfahan Fertility and Infertility center Semen samples were col lected on the day of oocyte recovery. Following routine semen analysis, maj or portion of tile semen was prepared for routine IVF insemination and the remaining was used for following sperm nuclear maturity tests: chromomycin A3 (CMA3), aniline blue, sodium dodecyl sulphate (SDS) test, and acridine o range test with or without heat shock (87 degreesC, 5 min). Sperms (200) we re evaluated for each test. Tile results were recorded and analyzed for the ir correlation to fertilization rate, using correlation coefficient, logist ic regression analysis, student t-test, and receiver operating characterist ics (ROC) curve. Results: Among these tests aniline blue and CMA3, and semen parameters, spe rm morphology, and sperm motility showed a significant correlation with fer tilization rate Using logistic regression analysis sperm morphology and CMA 3 were the only independent factors related to in vitro fertilization. ROC curves showed that among above tests, CMA3 is the most specific and sensiti ve for sperm nuclear maturity. Conclusion: Among CMA3, aniline blue, SDS test, and acridine orange CMA3 wa s the most sensitive and specific rest that can be used along with routine semen analysis for more precise prediction of fertilization rate.