T. Olivry et al., A spontaneous canine model of mucous membrane (cicatricial) pemphigoid, anautoimmune blistering disease affecting mucosae and mucocutaneous junctions, J AUTOIMMUN, 16(4), 2001, pp. 411-421
Mucous membrane pemphigoid (MMP) is a rare autoimmune blistering dermatosis
of humans that was previously known as cicatricial pemphigoid. It is chara
cterized by vesicles, ulcers and scarring that affect predominantly mucosae
and mucocutaneous junctions. Circulating autoantibodies recognize epitopes
on basement membrane proteins such as collagen XVII or laminin-5/6. Herein
, we describe the clinico-pathological and immunological characteristics of
17 dogs afflicted with a dermatosis homologous to MMP of humans. Patients
exhibited vesicles and erosions predominantly on mucous membranes or mucocu
taneous junctions of the mouth, nose, eyes, genitalia or anus. Histopatholo
gy revealed subepithelial vesicles with variable dermal inflammation. Direc
t immunofluorescence demonstrated IgG or complement at the dermoepithelial
junction. Indirect immunofluorescence using salt-split epithelia permitted
the detection of circulating basement membrane-specific IgG autoantibodies
in 15 cases. In 11 patients, autoantibodies recognized the NC16A segment of
collagen XVII, as determined by salt-split indirect immunofluorescence, im
munoblotting using canine keratinocytes and ELISA with synthetic canine pep
tides. In one dog, autoantiodies bound to the dermal side of salt-split epi
thelia and recognized epitopes within the 30 kDa carboxyterminal segment of
human collagen XVII. Canine MMP, Like its human counterpart, exhibits dist
inctive clinical signs and histopathological lesions, yet circulating autoa
ntibodies target different antigenic epitopes. This spontaneous canine mode
l of MMP could prove useful for studies on the pathogenesis or therapy of t
his human disease. (C) 2001 Academic Press.