Gg. Chiang et Bm. Sefton, Specific dephosphorylation of the Lck tyrosine protein kinase at Tyr-394 by the SHP-1 protein-tyrosine phosphatase, J BIOL CHEM, 276(25), 2001, pp. 23173-23178
The protein-tyrosine phosphatase SHP-1 has been shown to be a negative regu
lator of multiple signaling pathways in hematopoietic cells. In this study,
we demonstrate that SHP-1 dephosphorylates the lymphoid-specific Src famil
y kinase Lck at Tyr-394 when both are transiently co-expressed in nonlympho
id cells. We also demonstrate that a GST-SHP-1 fusion protein specifically
dephosphorylates Lck at Tyr-394 in vitro. Because phosphorylation of Tyr-39
4 activates Lck, the fact that SHP-1 specifically dephosphorylates this sit
e suggests that SHP-1 is a negative regulator of Lck. The failure of SHP-1
to inactivate Lck may contribute to some of the lymphoid abnormalities obse
rved in motheaten mice.