Self-assembly and aggregation of guanine rich sequences can provide useful
insights into DNA nanotechnology and telomeric structure and function. In t
his paper, we designed a guanine rich sequence d(GGCGTTTTGCGG). We found th
at it can form stable structure in appropriate condition and it exhibits an
anomalous CD spectra. This structures can be imaged in ambient environment
with a Nanoscope III AFM (Digital Instruments). We found it forms branch s
tructure and long multistrand DNA nanowire after incubation at 37 degreesC
for 6-12 hours in 25 mM TE (pH=8.0) + 5 mM Mg2+ + 50 mM K+. The ability to
self-assemble into branches and long wires not only clearly demonstrate its
potential as scaffold structures for nanotechnology, but also give aids to
understand telomeric structure further. We have proposed a model to explai
n how these structures formed.