Cloning and high expression of catalase gene from Bacillus sp TE124

Authors
Ni, JF Tokuyama, S Sogabe, A Kawamura, Y Tahara, Y
Citation
Jf. Ni et al., Cloning and high expression of catalase gene from Bacillus sp TE124, J BIOSCI BI, 91(4), 2001, pp. 422-424
Citations number
16
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
13891723 → ACNP
Volume
91
Issue
4
Year of publication
2001
Pages
422 - 424
Database
ISI
SICI code
1389-1723(200104)91:4<422:CAHEOC>2.0.ZU;2-1
Abstract
An efficient expression system for producing catalase in Bacillus was devel oped. A catalase was purified from Bacillus sp. TE124 and the catalase gene was cloned by plaque hybridization with a probe constructed from the N-ter minal amino acid sequence of the enzyme. The gene, containing an open readi ng frame of 1452 bp, was subcloned into pHY300PLK for self-cloning into the organism. As a result, the production of catalase increased 20-fold over t hat of the parent strain.