Samples were subjected to continuous and step-pulsed pressurization, in bot
h cases at 7 and 40 degreesC. There was a reduction of microbial flora (tot
al viable count and lactic bacteria) after pressurization and during storag
e at 2.5 to 3.0 degreesC, chiefly in the lot pressurized by step-pulse at 4
00 MPa, 40 degreesC. Pressure-induced modification of the microbial flora r
esulted in a lower level of nitrogenous compounds. Pressurization reduced a
utolytic activity, but shear strength values remained stable throughout sto
rage. There was less drip loss in the pressurized lots at 7 degreesC that a
t 40 degreesC, and the WHC values decreased during storage. Shelf life of t
he pressurized octopus overall was 43 d longer than unpressurized.