Elevated levels of indicator bacteria within a river system represent a sig
nificant impairment to surface water quality in many urban areas within the
Great Lakes watershed. Outfalls from combined sewer systems are assumed to
be the major source of bacteria to streams in many of these urban areas, i
ncluding the Rouge River of southeastern Michigan. Current remediation stra
tegies largely disregard other potential sources, including water entering
the river from its headwaters region, rural runoff and contaminated groundw
ater. These other potential sources of bacterial pollution to the Rouge Riv
er were assessed and compared with bacteria contributed from combined sewer
outfalls (CSOs). Fecal coliform (FC) and fecal streptococci (FS) densities
were determined at 28 locations in the Rouge River Watershed during the sp
ring and summer of 1997 1998, and 1999. The mean levels of both indicator g
roups vary considerably along each branch of the river and show no correlat
ion to CSO locations. The magnitude of both FC and FS levels are, in fact,
frequently greater at sites upstream of the part of the river influenced by
discharges from CSOs. The highest FC levels coincided with rainfall events
, but FC levels at various sites along each branch of the Rouge River viola
ted acceptable water quality standards (200 colonies/100 mL) at nearly 50%
of the sites even during dry, weather. Total suspended solids were moderate
ly correlated with FC (r = 0.79) throughout the watershed, suggesting that
solids may play a role in transporting bacteria into the Rouge River. The d
ata depict a strong influence of upstream water and rural runoff on the wat
er quality of the Rouge River. FC to FS ratios (FC/FS) suggest the primary
source of bacteria throughout the watershed is from domesticated animals an
d wildlife and not from sewage derived from CSOs.