Genomic identity of pyelonephritogenic Escherichia coli isolated from blood, urine and faeces of children with urosepsis

Chromosomal genotypes of Escherichia coli isolates from blood, urine and fa eces of infants with urosepsis were studied to find possible clonality of t he isolates. The isolates were analysed by PCR for class I, II and III alle les of the pyelonephritis-associated adhesin gene papG, The macrorestrictio n profiles of the papG-positive isolates were analysed by pulsed-field gel electrophoresis and their O serogroups were determined. Genetically identic al E. coli isolates from the blood, urine and faeces of the same infant wer e found in 8 of 10 infants. This finding confirmed the results of previous phenotypic studies that the reservoir of pyelonephritogenic E. coli is inde ed the colon.