CLONING OF CDNA FOR GOLDFISH ACTIVIN BETA(B) SUBUNIT, AND THE EXPRESSION OF ITS MESSENGER-RNA IN GONADAL AND NON-GONADAL TISSUES

Authors
GE W MIURA T KOBAYASHI H PETER RE NAGAHAMA Y
Citation
W. Ge et al., CLONING OF CDNA FOR GOLDFISH ACTIVIN BETA(B) SUBUNIT, AND THE EXPRESSION OF ITS MESSENGER-RNA IN GONADAL AND NON-GONADAL TISSUES, Journal of molecular endocrinology, 19(1), 1997, pp. 37-45
Citations number
30
Categorie Soggetti
Endocrynology & Metabolism
Journal title
Journal of molecular endocrinology → ACNP
ISSN journal
09525041
Volume
19
Issue
1
Year of publication
1997
Pages
37 - 45
Database
ISI
SICI code
0952-5041(1997)19:1<37:COCFGA>2.0.ZU;2-4
Abstract
We have cloned a full length cDNA coding for activin beta(B) subunit f rom the goldfish ovary. Sequence analysis of the goldfish activin beta (B) shows that this peptide is extremely conserved across vertebrates. The mature region of goldfish activin beta(B) has 93 and 98% amino ac id identity with that of human and zebrafish beta(B) subunit respectiv ely. The identity of the cloned goldfish activin beta(B) was further c onfirmed by expressing the protein in the Chinese hamster ovary (CHO) cells followed by detection of the specific activity of activin in the culture medium using F5-5 cell assay. mRNA of goldfish activin beta(B ) is expressed in a variety of goldfish tissues including ovary, testi s, brain, pituitary, kidney and liver, suggesting a wide range of phys iological roles for activin in the goldfish.