Peroxiredoxin in bovine ocular tissues: Immunohistochemical localization and in situ hybridization

Peroxiredoxins are widely distributed in nature and constitute a molecular family of antioxidant enzymes which decompose hydrogen peroxide and alkyl h ydroperoxides. We have previously characterized a peroxiredoxin from bovine ciliary body and deduced its amino acid sequence from analysis of cDNA clo nes encoding the protein. In this work, we investigated the immunolocalizat ion of this novel antioxidant enzyme and its mRNA expression in bovine eye tissues. High levels of immunoreactivity and mRNA for the enzyme were detec ted in corneal epithelium. Distinct immunoreactivity and mRNA expression fo r peroxiredoxin were also detected in uveal tissues, some of the retinal ce ll layers and ocular vasculature.