The conformational profile of the conformationally constrained cyclohexane
analogs of phenylalanine (1-amino-2-phenylcyclohexanecarboxylic acids, c(6)
Phe) was assessed using computational methods. For this purpose, the confor
mational space of the N-acetyl methylamide derivatives of the stereoisomers
(2S,3R)c(6)Phe and (ZS,3S)c(6)Phe was explored by computing their respecti
ve Ramachandran maps, and low-energy minima were characterized at molecular
mechanics level by means of the AMBER program, using the parm94 force fiel
d set of parameters. In order to assess the performance of the molecular me
chanics calculations, each of the low-energy conformations was also investi
gated further at the ab initio level. Accordingly, the molecular mechanics
geometries were used as starting conformations to perform full geometry opt
imizations at the Hartree-Fock level, using a 6-31G(d) basis set. Analysis
of the results revealed that the cyclohexane structure directly induces som
e restrictions on the backbone, and constrains the orientation of the aroma
tic side-chain to two narrow regions for each stereoisomer, The conformatio
nal profile of these amino acids is then explained on the grounds of the in
teraction between the rigidly held phenyl ring and the main chain NH and CO
groups. The results obtained are in good accordance with the experimental
observations.