In vitro comparison of bioabsorbable and non-resorbable membranes in bone regeneration

Citation
L. Marinucci et al., In vitro comparison of bioabsorbable and non-resorbable membranes in bone regeneration, J PERIODONT, 72(6), 2001, pp. 753-759
Citations number
45
Categorie Soggetti
Dentistry/Oral Surgery & Medicine","da verificare
Journal title
JOURNAL OF PERIODONTOLOGY
ISSN journal
00223492 → ACNP
Volume
72
Issue
6
Year of publication
2001
Pages
753 - 759
Database
ISI
SICI code
0022-3492(200106)72:6<753:IVCOBA>2.0.ZU;2-Z
Abstract
Background: Barrier membranes are used to prevent downgrowth of the oral mu cosa along the root surface and to allow alveolar bone regeneration in guid ed tissue regeneration. Several studies have demonstrated bone regenerates in the presence of bioabsorbable and non-resorbable membranes, but no studi es have compared multiple bioabsorbable barriers to one another and to non- resorbable barriers. This study evaluated the in vitro influence of bioabso rbable and non-resorbable membranes on specific parameters of human osteobl ast activity. Methods: Human osteoblasts were cultured on bioabsorbable membranes made of collagen, hyaluronic acid, and poly DL-lactide, and the most common non-re sorbable membrane which is made of expanded polytetrafluoroethylene (ePTFE) . The osteoblasts were cultured in vitro for 24 hours on barrier membranes in the presence of H-3-thymidine and H-3-proline to study cell proliferatio n and collagen synthesis. Transforming growth factor-pi (TGF-PI) secretion was evaluated in conditioned media using an ELISA kit. Results: The results showed that collagen and poly DL-lactide stimulated DN A synthesis more than ePTFE and hyaluronic acid. All bioabsorbable membrane s significantly increased collagen synthesis and alkaline phosphatase activ ity. Collagen and hyaluronic acid increased secretion of TGF-beta (1), a gr owth factor involved in bone remodeling. Conclusions: These data suggest bioabsorbable membranes, particularly colla gen and hyaluronic acid, may promote bone regeneration through their activi ty on osteoblasts.