LC determination of atropine sulfate and scopolamine hydrobromide in pharmaceuticals

An accurate, simple, reproducible and sensitive method for the determinatio n of atropine sulfate and scopolamine hydrobromide has been developed and v alidated. Atropine sulfate and scopolamine hydrobromide were separated usin g a mu Bondapack C-18 column by isocratic elution with flow rate 1.0 ml/min . The mobile phase composition was methanol, water, formic acid (165:35:1; v/v/v) and pH adjusted 8.3 with triethylamine. The samples were detected at 230 nm using photo-diode array detector. The linear range of detection for atropine sulfate (I) and scopolamine hydrobromide (II) were between 10.38 and 1038 mug/ml with a limit of quantification (LOQ) of 10.38, 10.00 and 10 34 mug/ml with an LOQ of 10.00 mug/ml respectively. The linearity, range, p eak purity, selectivity, system performance parameters, precision, accuracy , robustness and ruggedness for (I) and (II) were also shown acceptable val ues. (C) 2001 Elsevier Science B.V. All rights reserved.