FIA of vitamin C in blood serum in humans at increasing ethanol concentration

The evaluation of the ethanol influence on the changes in vitamin C levels has been done in humans at increasing ethanol concentration. Flow injection analysis method (FIA) with the spectrophotometric detection was used to de termine L-ascorbic acid levels. The procedure is based on the oxidation of the analysis with iron (III) and 2,2 ' -dipyrydyl. Concentrations of vitami n C in the range of 0.4-1.3 ppm have been determined with a relative standa rd deviation (R.S.D.) 1.2% (n = 15). The injection rate was 40 samples h(-1 ). The comparison of L-ascorbic acid levels in humans showed a statistical difference at P < 0.05 in the analysed group. (C) 2001 Elsevier Science B.V . All rights reserved.