Zl. Cai et al., Electron spin resonance study of electron and hole transfer in DNA: Effects of hydration, aliphatic amine cations, and histone proteins, J PHYS CH B, 105(25), 2001, pp. 6031-6041
In this work, we employ election spin resonance spectroscopy to investigate
the effects of hydration, and various cationic complexing agents, such as,
aliphatic amine cations and histone proteins, on electron and hole transfe
r in DNA. Electrons and holes generated by irradiation at 77 K are trapped
on DNA and transfer to a randomly interspersed intercalator, mitoxantrone (
MX). Monitoring the changes of ESR signals of MX radicals, one electron oxi
dized guanine (G(.+)), one-electron reduced cytosine [C(N3)H-.], and thymin
e anion radicals (T.-) with time at 77 K allows for the direct observation
of electron and hole transfer. The apparent transfer distance (D-a) in bps
is derived from the change in radicals with time and is a measure of the to
tal number of bps within the tunneling range. In all solid DNA samples in w
hich tunneling from electrons and holes to an intercalator was investigated
, we find that the distance between DNA duplexes is the dominant factor in
the degree of transfer observed. Tn hydrated DNA samples intercalated with
MX, the apparent distances and rates of hole and electron transfer to MX de
crease as hydration level increases mainly because the distance between DNA
duplexes increases with hydration. DNA complexing agents such as poly-lysi
ne, polyethylenimine, nucleohistone, and cationic lipids also reduce the ap
parent transfer rates by reducing the amount of transfer between duplexes.
Transfer rates in DNA complexed with spermine, however, are similar to thos
e in equivalently hydrated MX-DNA. A double layer of cationic lipids is fou
nd to nearly isolate DNA duplexes from electron or hole transfer to adjacen
t duplexes. Our modeling of rates and distances of electron transfer in DNA
-complexes allow for estimates of the spacing between DNA duplexes in each
complex.