Fq. Guo et al., REE BOUND PROTEINS IN NATURAL PLANT FERN DICRANOPTERIS-DICHITOMA BY MAA, Journal of radioanalytical and nuclear chemistry, 209(1), 1996, pp. 91-99
Biochemical techniques, including pH variation, outsalting, ultracentr
ifugation, gel filtration chromatography and electrophoresis, etc., ha
ve been employed together with instrumental neutron activation analysi
s (INAA) to study the rare earth elements (REE) bound proteins in the
natural plant fern, Dicranopteris dichitoma. INAA was also used to ide
ntify whether the proteins were bound firmly with REE,The results obta
ined show that two REE bound proteins (REP-I and REP-II) have been sep
arated. The molecular weight of REP-I on Sephadex G-200 gel column is
about 8.10(5) Daltons and that of REP-II is less than 12,400 Daltons,
respectively. However, SDS-PAGE of the two proteins shows that they ma
inly have two protein subunits with MW 14,100 and 38,700 Daltons. They
are probably conjugated proteins, glycoproteins with different glyco-
units.