Application of ion-impact energy measurement to electrospray ionization mass spectrometry of proteins and protein mixtures

Authors
Rabin, MW Hilton, GC Martinis, JM
Citation
Mw. Rabin et al., Application of ion-impact energy measurement to electrospray ionization mass spectrometry of proteins and protein mixtures, J AM SOC M, 12(7), 2001, pp. 826-831
Citations number
15
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
ISSN journal
10440305 → ACNP
Volume
12
Issue
7
Year of publication
2001
Pages
826 - 831
Database
ISI
SICI code
1044-0305(200107)12:7<826:AOIEMT>2.0.ZU;2-R
Abstract
We have used a normal metal-insulator-superconductor (NIS) microcalorimeter to measure the impact energy of protein ions produced by electrospray ioni zation (ESI) in a magnetic-sector mass: spectrometer (MS). We have used the se measurements to resolve spectral ambiguities and to analyze protein mixt ures. Energy measurement may be useful for the direct MS analysis of comple x biopolymer mixtures that normally would confound ESI-MS deconvolution alg orithms. Published by Elsevier Science Inc.