Many nonmammalian cells exhibit postentry blocks to transduction by gammaretroviruses pseudotyped with various viral envelopes, including vesicular stomatitis virus G glycoprotein
C. Dirks et Ad. Miller, Many nonmammalian cells exhibit postentry blocks to transduction by gammaretroviruses pseudotyped with various viral envelopes, including vesicular stomatitis virus G glycoprotein, J VIROLOGY, 75(14), 2001, pp. 6375-6383
Previous studies have suggested that Moloney murine leukemia virus (MoMLV)-
based vectors pseudotyped with the vesicular stomatitis virus G glycoprotei
n (VSV-G) have extensive ability to transduce nonmammalian cells. However,
we have identified multiple cell lines from fish (FHM), mosquitoes (Mos-55)
, moths (Sf9 and High-5), flies (S2), and frogs (XPK2) that are not efficie
ntly transduced by MoMLV-based vectors pseudotyped,vith many different vira
l envelope proteins, including VSV-G, while the same vectors are functional
in these cells following transfection, A comparison of MoMLV-based vector
transduction in mammalian and nonmammalian cells shows that the nonmammalia
n cells exhibit blocks at either entry, reverse transcription, or integrati
on. Additionally, VSV-G-pseudotyped MoMLV-based vector transduction is atte
nuated in the zebrafish cell line ZF4 at entry and/or reverse transcription
, whereas other transduction processes are unaffected. We show that the var
iation of transduction by MoMLV-based vectors in mammalian and nonmammalian
cells is not due to differences in culture conditions or cell division rat
e but is likely the result of divergence in cellular factors required for r
etroviral transduction.