Risk of trisomy 21 in offspring of patients with Klinefelter's syndrome

Intracytoplasmic sperm injection (ICSI) has given some patients with Klinef elter's syndrome (ie, men with an XXY sex-chromosome profile) the chance to become fathers, but the genetic makeup of the spermatozoa used for the inj ection is a concern. We studied the segregation of the sex chromosomes and chromosomes 1 and 21 by multicolour fluorescence in-situ hybridisation in a patient with non-mosaic Klinefelter's syndrome who was a candidate for ICS I. As other workers have found, we saw a higher rate of 24,XX and 24,XY spe rmatozoa in the patient than in controls. However, we also found a much hig her frequency of disomy 21 in the spermatozoa of this patient than in contr ols (6.2 vs 0.4%). Any child conceived by ICSI using this man's sperm will thus have a proportionally higher risk of trisomy 21.