Macrolide resistance in Streptococcus pyogenes isolates from throat infections in the region of Aachen, Germany

Citation
Cm. Brandt et al., Macrolide resistance in Streptococcus pyogenes isolates from throat infections in the region of Aachen, Germany, MICROB DR R, 7(2), 2001, pp. 165-170
Citations number
39
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
MICROBIAL DRUG RESISTANCE-MECHANISMS EPIDEMIOLOGY AND DISEASE
ISSN journal
10766294 → ACNP
Volume
7
Issue
2
Year of publication
2001
Pages
165 - 170
Database
ISI
SICI code
1076-6294(200122)7:2<165:MRISPI>2.0.ZU;2-5
Abstract
Macrolide-resistance was assessed in 216 consecutive Streptococcus pyogenes isolates from throat infections in the region of Aachen, Germany. Seventee n isolates were resistant to erythromycin: 12 isolates revealed a macrolide (M) phenotype and harbored mefA, and five strains expressed an inducible m acrolide-lincosamide-streptogramin B (MLSB) phenotype of which four strains harbored ermA(TR) and one strain contained ermB(AM), Telithromycin (HMR 36 47) and quinupristin/dalfopristin remained active particularly against the ermA(TR)-containing S, pyogenes isolates studied. Random amplified polymorp hic DNA analysis identified multiple clones among erythromycin-resistant st rains, but did not discriminate beyond the emm-type, mefA was present in th ree isolates either with emm2, emm12, or emm75, and in nine isolates with e mm4. All four strains with ermA(TR) contained emm77, and the single strain with ermB(AM) harbored emm1, Despite the relative low rate of macrolide-res istance, these data suggest that at least three different macrolide-resista nce determinants are prevalent in Germany and that mefA has spread rapidly into multiple clones of S, pyogenes.