Analysis of fatty acid, and lipoprotein, metabolism by GC and HPLC: Effectof low-density lipoprotein apheresis

Blood samples from 5 hyperlipidemic patients on chronic treatment with low- density lipoprotein (LDL)- apheresis were analysed for lipids and fatty aci ds in serum, lipoprotein fractions and erythrocyte membrane by capillary ga s chromatography (GC), reversed-phase high-performance liquid chromatograph y (LC), spectrofluorometry and spectrophotometry. LDL-apheresis has been as sociated with significant changes of fatty acids metabolism in relation to triglyceride-rich lipoproteins. Oleic acid may exert its hypo-triglyceridem ic effect via VLDL, IDL, LDL and HDL Fractions. Polyunsaturated fatty acids , associated with triglyceride metabolism via IDL or VLDL, are linoleic, ga mma-linolenic and docosahexaenoic fatty acids.