Methods for the spiral Salmonella mutagenicity assay including specializedapplications

Citation
Ld. Claxton et al., Methods for the spiral Salmonella mutagenicity assay including specializedapplications, MUT RES-R M, 488(3), 2001, pp. 241-257
Citations number
101
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH
ISSN journal
13835742 → ACNP
Volume
488
Issue
3
Year of publication
2001
Pages
241 - 257
Database
ISI
SICI code
1383-5742(200106)488:3<241:MFTSSM>2.0.ZU;2-R
Abstract
An automated approach to bacterial mutagenicity testing - the spiral Salmon ella assay - was developed to simplify testing and to reduce the labor and materials required to generate dose-responsive mutagenicity information. Th is document provides the reader with an overview of the spiral assay and a discussion of its application for examining the mutagenic potential of pure compounds, complex environmental mixtures, and interactive effects. Guidel ines for performing a routine spiral assay are presented, and alternative t est methods intended to overcome a variety of technical difficulties (such as restricted sample availability, sample viscosity or volatility, etc.) ar e recommended. Methods for the computerized analysis of data and the interp retation of results are discussed. Published by Elsevier Science B.V.